Microbiological profile of raw refrigerated and processed bovine milk at dairy industries from Vale do Taquari, Rio Grande do Sul, Brazil

Milk is an essential food, widely consumed by the population. Brazil is one of the world's largest producers of milk. Milk quality is influenced by several factors in all its stages of production. The aim of this study was to determine the microbiological profile of refrigerated and processed raw bovine milk from industries in Vale do Taquari, state of Rio Grande do Sul, Brazil, using metagenomic analysis. A total of six samples were collected, one of refrigerated raw milk from the tanker truck, one of pasteurized milk and one of milk sterilized by the ultra-high temperature (UHT) process, in each of the industries. The identification of the milk microbiota was performed by sequencing the 16S rRNA gene. The results show that refrigerated raw milk has a greater number of microorganisms, followed by pasteurized milk and sterilized milk, successively. Processed milk showed the presence of beneficial microorganisms such as Streptococcus thermophilus and Streptococcus macedonicus. Nevertheless, even UHT milk showed the presence of microorganisms considered harmful, such as the Bacillus cereus group, Aeromonas dhakensis, Enterobacter bacterium and Acinetobacter haemolyticus. Metagenomics is a valuable tool for the thorough evaluation of the milk microbiota in order to implement the processing stages in industries.

A escrita como dispositivo na clínica da adolescência: a partir de um caso clínico / Writing as a device in the adolescence clinic: considerations from a clinical case

A adolescência é um momento em que a representação que sustentava o sujeito já não se encontra suficiente, convocando-o a transformações. Assim torna-se necessário para o adolescente o trabalho de ressignificação de si, a reconstrução das bordas que permitam o estabelecimento de novas relações consigo e com o mundo. O presente trabalho pretende discutir a temática da escrita enquanto dispositivo clínico, partindo do lugar ocupado pela escrita na história da psicanálise e no processo da adolescência, assim como de questões relacionadas às singularidades da clínica psicanalítica com adolescentes

Adolescence is a moment when the representation that sustained the subject is no longer enough, calling him to transformations. This, it becomes necessary for the adolescent the work of re-signification of himself, the reconstruction of the edges that allow the establishment of new relations with himself and with the world. The present work intends to discuss the theme of writing as a clinical device, starting from the place occupied by writing in the history of psychoanalysis and in the process of adolescence, as well as issues related to the singularities of the psychoanalitic clinic with adolescents